Characterizing the protein profiles of reactive astrocyte subtypes is crucial, given that proteins govern nearly all aspects of cell functions, including immunomodulation and metabolic signaling. In this context, I conducted proteomic profiling of a recently identified reactive astrocyte subtype termed "interferon-responsive reactive astrocytes" (IRRAs). My work identified over 2000 unique proteins within the cellular proteome, including several interferon-responsive proteins such as IFIT3, IFITM3, MX1, GBP2, and others. Additionally, I demonstrated the enrichment of IRRA markers in human AD postmortem brain tissue.
In another collaborative effort, I also showed that a subset of astrocytes in human AD brains exhibit enrichment for neurotoxic reactive astrocyte-specific proteins. Importantly, these markers were identified using induced pluripotent stem cell-derived astrocytes in this study.
In another collaborative effort, I also showed that a subset of astrocytes in human AD brains exhibit enrichment for neurotoxic reactive astrocyte-specific proteins. Importantly, these markers were identified using induced pluripotent stem cell-derived astrocytes in this study.